HITRAP CHELATING HP
产品名称： HITRAP CHELATING HP
英文名称： HITRAP CHELATING HP
产品编号： 17-0408-01
产品价格： 0
产品产地： USA
品牌商标： 美国GE
更新时间： null
使用范围： null
上海基星生物科技有限公司
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- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983 点击查看
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HiTrap™ Chelating HP Columns
- For purification of histidine-tagged* proteins using different kinds of metal ions†.
- For optimizing purification of (histidine)6-tagged protein purification when Ni2+ is not the best choice of metal ion.
- Prepacked with Chelating Sepharose™ High Performance.
- Purifying proteins with exposed histidine groups via metal ion complex formation.
- Iminidoacetic acid coupled to Sepharose High Performance.
HiTrap™ Chelating HP Columns
Technical Information
)
| TECHNICAL SPECIFICATIONS | |
| Chelating group | Iminodiacetic acid |
| Chelating group coupling linkage | Ether |
| Matrix | Highly cross-linked agarose, 6% |
| Average particle size | 34 μm |
| Metal ion capacity | » 23 μmol Cu2+/ml medium |
| Binding capacity | » 12 mg (histidine)6-tagged protein (Mr 27 600)/ml medium |
| Spacer arm | 7-carbon |
| pH stability | 3-13 (long term), 2-14 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
† For more information see Application Note 18-1145-18: Purification of (histidine)6-tagged proteins using HiTrap™ Chelating HP columns charged with different metal ions.